(AGENPARL) – LONDON (UNITED KINGDOM), lun 28 dicembre 2020
Light–Oxygen–Voltage (LOV) domains are bule light sensors and play an important role in signal transduction in many organisms. Generally, LOV domains use chromophores to absorb photons, and then photochemical reactions will occur to convert light energy into chemical energy and transduce it to the main chain of proteins. These reactions can cause conformational rearrangement of proteins, and thus leading to signal transduction. Therefore, it is important to study the signal transduction process of LOV domains for understanding the control mechanism of cellular function. However, how small photochemical changes in the active sites of the LOV domains lead to large conformational rearrangements of proteins, which in turn lead to signal transduction, it has been puzzling us for a long time. Currently, the LOV domains are mainly studied in plants. The signalling transduction mechanism of LOV domains in bacteria are still unclear. In this work, Markov state model (MSM) combined with molecular dynamics (MD) simulations were applied to investigate the signal transduction process of the LOV protein from pseudomonas putida (PpSB1-LOV). The present work will play an important role in understanding the signal transduction mechanism of PpSB1-LOV domain, which may provide theoretical basis for the design and improvement of the LOV-based optogenetic tools.
You have access to this article