(AGENPARL) – LONDON (UNITED KINGDOM), ven 25 settembre 2020
Pantetheinase is an amidohydrolase that cleaves pantetheine into pantothenic acid and cysteamine. Functional studies have found that ubiquitous expression of this enzyme is associated with many inflammation diseases. However, the lack of near-infrared fluorescence probes limits the better understanding of the functions of the enzyme. In this work, we have developed a new near-infrared fluorescence probe CYLP for bioimaging of pantetheinase by using pantothenic acid with a self-immolative linker as a recognition group. The probe produces sensitive fluorescence off-on response at 710 nm to pantetheinase with a detection limit of 0.02 ng/mL, and can be used to image the intraperitoneal pantetheinase activity in mice in vivo. Moreover, with the probe we have observed that pantetheinase is significantly increased in the tissues of mouse inflammatory models as well as in the intestines of the inflammatory bowel disease mice. Therefore, CYLP may provide a convenient and intuitive tool for studying the role of pantetheinase in diseases.
This article is Open Access